ARG2 ELISA Kit (Human)

CAT: 0247-OKCD01118-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD01118-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Arginase II (Arg2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Arginase II (Arg2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Arginase 2
Gene Aliases
Arginase II; arginase, type II; arginase-2, mitochondrial; kidney arginase; kidney-type arginase; L-arginine amidinohydrolase; L-arginine ureahydrolase; nonhepatic arginase; non-hepatic arginase; type II arginase.
Gene ID
384
Accession Number
NP_033835
Reactivity
Homo sapiens|Human
Target
May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS) . Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4 (+) and CD8 (+) T cells (PubMed:27745970) . May suppress inflammation-related signaling in asthmatic airway epithelium (PubMed:27214549) . May contribute to the immune evasion of H.pylori by restricting M1 macrophage activation and polyamine metabolism (By similarity) . In fetal dendritic cells may play a role in promoting immune suppression and T cell TNF-alpha production during gestation (PubMed:28614294) . Regulates RPS6KB1 signaling, which promotes endothelial cell senescence and inflammation and implicates NOS3/eNOS dysfunction (PubMed:22928666) . Can inhibit endothelial autophagy independently of its enzymatic activity implicating mTORC2 signaling (PubMed:25484082) . Involved in vascular smooth muscle cell senescence and apoptosis independently of its enzymatic activity (PubMed:23832324) . Since NOS is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase-2 plays a role in both male and female sexual arousal (PubMed:12859189) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginase II (Arg2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arginase II (Arg2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arginase II (Arg2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Arginase II (Arg2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
3.12-200ng/mL
Recovery
Matrix Recovery Range (%) Average (%) serum (n=5) 94-101 98 EDTA plasma (n=5) 86-101 93 heparin plasma (n=5) 94-101 98 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 94-101 98 serum (n=5) 94-101 98 EDTA plasma (n=5) 86-101 93 EDTA plasma (n=5) 86-101 93 heparin plasma (n=5) 94-101 98 heparin plasma (n=5) 94-101 98
Sensitivity
< 1.17 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-ARG2 Microplate 96 Wells (12 x 8 Well Strips) ARG2 Lyophilized Standard 2 x 200 ng 100X Biotinylated ARG2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-ARG2 Microplate 96 Wells (12 x 8 Well Strips) Anti-ARG2 Microplate 96 Wells (12 x 8 Well Strips) ARG2 Lyophilized Standard 2 x 200 ng ARG2 Lyophilized Standard 2 x 200 ng 100X Biotinylated ARG2 Detector Antibody 120 uL 100X Biotinylated ARG2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBZI9
Specificity
This assay has high sensitivity and excellent specificity for detection of Arginase II (Arg2) . No significant cross-reactivity or interference between Arginase II (Arg2) and analogues was observed.
Protein Length
354
NCBI Gene Symbol
ARG2
Protein Name
Arginase-2, mitochondrial
Gene Name URL
ARG2
Nucleotide Accession Number
NM_009705.3

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