ARG2 ELISA Kit (Human)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Arginase II (Arg2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Arginase II (Arg2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Arginase 2

• Gene Aliases:

  Arginase II; arginase, type II; arginase-2, mitochondrial; kidney arginase; kidney-type arginase; L-arginine amidinohydrolase; L-arginine ureahydrolase; nonhepatic arginase; non-hepatic arginase; type II arginase.

• Gene ID:

  384

• Accession Number:

  NP_033835

• Reactivity:

  Homo sapiens|Human

• Target:

  May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS) . Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4 (+) and CD8 (+) T cells (PubMed:27745970) . May suppress inflammation-related signaling in asthmatic airway epithelium (PubMed:27214549) . May contribute to the immune evasion of H.pylori by restricting M1 macrophage activation and polyamine metabolism (By similarity) . In fetal dendritic cells may play a role in promoting immune suppression and T cell TNF-alpha production during gestation (PubMed:28614294) . Regulates RPS6KB1 signaling, which promotes endothelial cell senescence and inflammation and implicates NOS3/eNOS dysfunction (PubMed:22928666) . Can inhibit endothelial autophagy independently of its enzymatic activity implicating mTORC2 signaling (PubMed:25484082) . Involved in vascular smooth muscle cell senescence and apoptosis independently of its enzymatic activity (PubMed:23832324) . Since NOS is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase-2 plays a role in both male and female sexual arousal (PubMed:12859189) .

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginase II (Arg2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arginase II (Arg2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arginase II (Arg2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Arginase II (Arg2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  3.12-200ng/mL

• Recovery:

  Matrix Recovery Range (%) Average (%) serum (n=5) 94-101 98 EDTA plasma (n=5) 86-101 93 heparin plasma (n=5) 94-101 98 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 94-101 98 serum (n=5) 94-101 98 EDTA plasma (n=5) 86-101 93 EDTA plasma (n=5) 86-101 93 heparin plasma (n=5) 94-101 98 heparin plasma (n=5) 94-101 98

• Sensitivity:

  < 1.17 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-ARG2 Microplate 96 Wells (12 x 8 Well Strips) ARG2 Lyophilized Standard 2 x 200 ng 100X Biotinylated ARG2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-ARG2 Microplate 96 Wells (12 x 8 Well Strips) Anti-ARG2 Microplate 96 Wells (12 x 8 Well Strips) ARG2 Lyophilized Standard 2 x 200 ng ARG2 Lyophilized Standard 2 x 200 ng 100X Biotinylated ARG2 Detector Antibody 120 uL 100X Biotinylated ARG2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes:

  Formerly GWB-KBBZI9

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Arginase II (Arg2) . No significant cross-reactivity or interference between Arginase II (Arg2) and analogues was observed.

• Protein Length:

  354

• NCBI Gene Symbol:

  ARG2

• Protein Name:

  Arginase-2, mitochondrial

• Gene Name URL:

  ARG2

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_009705.3