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RGCC ELISA Kit (Human)

CAT: 0247-OKCD01083-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01083-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Response Gene To Complement 32 (RGC32) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Response Gene To Complement 32 (RGC32) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Regulator of cell cycle
Gene Aliases
BA157L14.2; C13orf15; regulator of cell cycle RGCC; response gene to complement 32 protein; RGC32; RGC-32.
Gene ID
28984
Accession Number
NP_054778
Reactivity
Homo sapiens|Human
Target
Modulates the activity of cell cycle-specific kinases. Enhances CDK1 activity. May contribute to the regulation of the cell cycle. May inhibit growth of glioma cells by promoting arrest of mitotic progression at the G2/M transition. Fibrogenic factor contributing to the pathogenesis of renal fibrosis through fibroblast activation.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Response Gene To Complement 32 (RGC32) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Response Gene To Complement 32 (RGC32) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Response Gene To Complement 32 (RGC32), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Response Gene To Complement 32 (RGC32) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
< 0.128 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-RGCC Microplate 96 Wells (12 x 8 Well Strips) RGCC Lyophilized Standard 2 x 20 ng 100X Biotinylated RGCC Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-RGCC Microplate 96 Wells (12 x 8 Well Strips) Anti-RGCC Microplate 96 Wells (12 x 8 Well Strips) RGCC Lyophilized Standard 2 x 20 ng RGCC Lyophilized Standard 2 x 20 ng 100X Biotinylated RGCC Detector Antibody 120 uL 100X Biotinylated RGCC Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBJD1
Specificity
This assay has high sensitivity and excellent specificity for detection of Response Gene To Complement 32 (RGC32) . No significant cross-reactivity or interference between Response Gene To Complement 32 (RGC32) and analogues was observed.
Protein Length
137
NCBI Gene Symbol
RGCC
Protein Name
Regulator of cell cycle RGCC
Gene Name URL
RGCC
Nucleotide Accession Number
NM_014059.2

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