DYNC1H1 ELISA Kit (Human)

CAT: 0247-OKCD01052-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD01052-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Dynein cytoplasmic 1 heavy chain 1
Gene Aliases
CMT2O; cytoplasmic dynein 1 heavy chain 1; Cytoplasmic dynein heavy chain 1; DHC1; DHC1a; DNCH1; Dnchc1; DNCL; DNECL; DYHC; dynein heavy chain, cytosolic; dynein, cytoplasmic, heavy polypeptide 1; HL-3; p22; SMALED1.
Gene ID
1778
Accession Number
NP_001367
Reactivity
Homo sapiens|Human
Target
Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Plays a role in mitotic spindle assembly and metaphase plate congression (PubMed:27462074) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
< 0.113 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-DYNC1H1 Microplate 96 Wells (12 x 8 Well Strips) DYNC1H1 Lyophilized Standard 2 x 20 ng 100X Biotinylated DYNC1H1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-DYNC1H1 Microplate 96 Wells (12 x 8 Well Strips) Anti-DYNC1H1 Microplate 96 Wells (12 x 8 Well Strips) DYNC1H1 Lyophilized Standard 2 x 20 ng DYNC1H1 Lyophilized Standard 2 x 20 ng 100X Biotinylated DYNC1H1 Detector Antibody 120 uL 100X Biotinylated DYNC1H1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBLF4
Specificity
This assay has high sensitivity and excellent specificity for detection of Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) . No significant cross-reactivity or interference between Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) and analogues was observed.
Protein Length
4646
NCBI Gene Symbol
DYNC1H1
Protein Name
Cytoplasmic dynein 1 heavy chain 1
Gene Name URL
DYNC1H1
Nucleotide Accession Number
NM_001376.4

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