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DNAH11 ELISA Kit (Human)

CAT: 0247-OKCD01051-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01051-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Dynein, Axonemal, Heavy Chain 11 (DNAH11) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Dynein, Axonemal, Heavy Chain 11 (DNAH11) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Dynein axonemal heavy chain 11
Gene Aliases
Axonemal beta dynein heavy chain 11; axonemal dynein heavy chain 11; CILD7; ciliary dynein heavy chain 11; DNAHBL; DNAHC11; DNHBL; DPL11; dynein axonemal heavy chain 11; dynein heavy chain 11, axonemal; dynein, axonemal, heavy polypeptide 11; dynein, ciliary, heavy chain 11.
Gene ID
8701
Accession Number
NP_001264044
Reactivity
Homo sapiens|Human
Target
Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynein, Axonemal, Heavy Chain 11 (DNAH11) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dynein, Axonemal, Heavy Chain 11 (DNAH11) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dynein, Axonemal, Heavy Chain 11 (DNAH11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dynein, Axonemal, Heavy Chain 11 (DNAH11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
< 0.127 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-DNAH11 Microplate 96 Wells (12 x 8 Well Strips) DNAH11 Lyophilized Standard 2 x 20 ng 100X Biotinylated DNAH11 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-DNAH11 Microplate 96 Wells (12 x 8 Well Strips) Anti-DNAH11 Microplate 96 Wells (12 x 8 Well Strips) DNAH11 Lyophilized Standard 2 x 20 ng DNAH11 Lyophilized Standard 2 x 20 ng 100X Biotinylated DNAH11 Detector Antibody 120 uL 100X Biotinylated DNAH11 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBGZ5
Specificity
This assay has high sensitivity and excellent specificity for detection of Dynein, Axonemal, Heavy Chain 11 (DNAH11) . No significant cross-reactivity or interference between Dynein, Axonemal, Heavy Chain 11 (DNAH11) and analogues was observed.
Protein Length
4516
NCBI Gene Symbol
DNAH11
Protein Name
Dynein heavy chain 11, axonemal
Gene Name URL
DNAH11
Nucleotide Accession Number
NM_001277115.1

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