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SH3BP2 ELISA Kit (Human)

CAT: 0247-OKCD01026-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01026-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level SH3 Domain Binding Protein 2 (SH3BP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level SH3 Domain Binding Protein 2 (SH3BP2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
SH3 domain binding protein 2
Gene Aliases
3BP2;3BP-2; Abl-SH3 binding protein 2; CRBM; CRPM; epididymis secretory sperm binding protein; RES4-23; SH3 domain-binding protein 2; TNFAIP3 interacting protein 2.
Gene ID
6452
Accession Number
NP_001116153
Reactivity
Homo sapiens|Human
Target
Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. Binds to phosphatidylinositols; linking the hemopoietic tyrosine kinase fes to the cytoplasmic membrane in a phosphorylation dependent mechanism.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SH3 Domain Binding Protein 2 (SH3BP2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SH3 Domain Binding Protein 2 (SH3BP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SH3 Domain Binding Protein 2 (SH3BP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SH3 Domain Binding Protein 2 (SH3BP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
< 0.126 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-SH3BP2 Microplate 96 Wells (12 x 8 Well Strips) SH3BP2 Lyophilized Standard 2 x 20 ng 100X Biotinylated SH3BP2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-SH3BP2 Microplate 96 Wells (12 x 8 Well Strips) Anti-SH3BP2 Microplate 96 Wells (12 x 8 Well Strips) SH3BP2 Lyophilized Standard 2 x 20 ng SH3BP2 Lyophilized Standard 2 x 20 ng 100X Biotinylated SH3BP2 Detector Antibody 120 uL 100X Biotinylated SH3BP2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBAR7
Specificity
This assay has high sensitivity and excellent specificity for detection of SH3 Domain Binding Protein 2 (SH3BP2) . No significant cross-reactivity or interference between SH3 Domain Binding Protein 2 (SH3BP2) and analogues was observed.
Protein Length
561
NCBI Gene Symbol
SH3BP2
Protein Name
SH3 domain-binding protein 2
Gene Name URL
SH3BP2
Nucleotide Accession Number
NM_001122681.1

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