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TRIP6 ELISA Kit (Mouse)

CAT: 0247-OKCD01015-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01015-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Thyroid Hormone Receptor Interactor 6 (TRIP6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Thyroid Hormone Receptor Interactor 6 (TRIP6) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Thyroid hormone receptor interactor 6
Gene Aliases
Thyroid receptor-interacting protein 6; TR-interacting protein 6; TRIP-6; ZRP-1; zyxin-related protein 1.
Gene ID
22051
Accession Number
NP_035769
Reactivity
Mouse|Mus musculus
Target
Relays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhesion and migration. Acts as a transcriptional coactivator for NF-kappa-B and JUN, and mediates the transrepression of these transcription factors induced by glucocorticoid receptor (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroid Hormone Receptor Interactor 6 (TRIP6) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyroid Hormone Receptor Interactor 6 (TRIP6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroid Hormone Receptor Interactor 6 (TRIP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thyroid Hormone Receptor Interactor 6 (TRIP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
< 0.144 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Trip6 Microplate 96 Wells (12 x 8 Well Strips) Trip6 Lyophilized Standard 2 x 20 ng 100X Biotinylated Trip6 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Trip6 Microplate 96 Wells (12 x 8 Well Strips) Anti-Trip6 Microplate 96 Wells (12 x 8 Well Strips) Trip6 Lyophilized Standard 2 x 20 ng Trip6 Lyophilized Standard 2 x 20 ng 100X Biotinylated Trip6 Detector Antibody 120 uL 100X Biotinylated Trip6 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBUV1
Specificity
This assay has high sensitivity and excellent specificity for detection of Thyroid Hormone Receptor Interactor 6 (TRIP6) . No significant cross-reactivity or interference between Thyroid Hormone Receptor Interactor 6 (TRIP6) and analogues was observed.
Protein Length
480
NCBI Gene Symbol
Trip6
Protein Name
Thyroid receptor-interacting protein 6
Gene Name URL
Trip6
Nucleotide Accession Number
NM_011639.3

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