TMOD3 ELISA Kit (Mouse)

CAT: 0247-OKCD01008-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD01008-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Tropomodulin 3 (TMOD3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Tropomodulin 3 (TMOD3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Tropomodulin 3
Gene Aliases
Tropomodulin 3; ubiquitous tropomodulin; tropomodulin-3; ubiquitous tropomodulin; U-Tmo; UTMO; U-Tmod; UTMOD.
Gene ID
50875
Accession Number
NP_058659
Reactivity
Mouse|Mus musculus
Target
Blocks the elongation and depolymerization of the actin filaments at the pointed end. The Tmod/TM complex contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane skeleton (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tropomodulin 3 (TMOD3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tropomodulin 3 (TMOD3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tropomodulin 3 (TMOD3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tropomodulin 3 (TMOD3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
< 0.108 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Tmod3 Microplate 96 Wells (12 x 8 Well Strips) Tmod3 Lyophilized Standard 2 x 20 ng 100X Biotinylated Tmod3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Tmod3 Microplate 96 Wells (12 x 8 Well Strips) Anti-Tmod3 Microplate 96 Wells (12 x 8 Well Strips) Tmod3 Lyophilized Standard 2 x 20 ng Tmod3 Lyophilized Standard 2 x 20 ng 100X Biotinylated Tmod3 Detector Antibody 120 uL 100X Biotinylated Tmod3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBRT5
Specificity
This assay has high sensitivity and excellent specificity for detection of Tropomodulin 3 (TMOD3) . No significant cross-reactivity or interference between Tropomodulin 3 (TMOD3) and analogues was observed.
Protein Length
352
NCBI Gene Symbol
Tmod3
Protein Name
Tropomodulin-3
Gene Name URL
Tmod3
Nucleotide Accession Number
NM_016963.2

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