SYNE1 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Nesprin 1 (Nesp1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Nesprin 1 (Nesp1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

7732-18-5

Spectrin repeat containing nuclear envelope protein 1

8B; AMC3; AMCM; ARCA1; C6orf98; CPG2; CPG2 full length; dJ45H2.2; EDMD4; enaptin; KASH domain-containing protein 1; KASH1; MYNE1; myocyte nuclear envelope protein 1; Nesp1; nesprin 1; nesprin-1; Nuclear envelope spectrin repeat protein 1; SCAR8; spectrin repeat containing, nuclear envelope 1; synaptic nuclear envelope protein 1; synaptic nuclei expressed gene 1.

23345

NP_149062

Homo sapiens|Human

Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment and nuclear migration in neural progenitors implicating LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes; SYNE1 and SYNE2 may act redundantly. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenenis; a probable SUN3:SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette.

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nesprin 1 (Nesp1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nesprin 1 (Nesp1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nesprin 1 (Nesp1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nesprin 1 (Nesp1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Tissue homogenates and other biological fluids.

0.312-20ng/mL

< 0.117 ng/mL

2°C to 8°C|-20°C

Component Amount _x005F Anti-SYNE1 Microplate 96 Wells (12 x 8 Well Strips) _x005F SYNE1 Lyophilized Standard 2 x 20 ng _x005F 100X Biotinylated SYNE1 Detector Antibody 120 uL _x005F Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount Component Amount Anti-SYNE1 Microplate 96 Wells (12 x 8 Well Strips) Anti-SYNE1 Microplate 96 Wells (12 x 8 Well Strips) SYNE1 Lyophilized Standard 2 x 20 ng SYNE1 Lyophilized Standard 2 x 20 ng 100X Biotinylated SYNE1 Detector Antibody 120 uL 100X Biotinylated SYNE1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

Formerly GWB-KBBGO5

This assay has high sensitivity and excellent specificity for detection of Nesprin 1 (Nesp1) ._x005F No significant cross-reactivity or interference between Nesprin 1 (Nesp1) and analogues was observed.

8797

SYNE1

Nesprin-1

SYNE1