TMPRSS2 ELISA Kit (Mouse)

• Reproducibility :

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Transmembrane Protease, Serine 2 (TMPRSS2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Transmembrane Protease, Serine 2 (TMPRSS2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name :

  Transmembrane protease, serine 2

• Gene Aliases :

  D16Ertd61; D16Ertd61e; epitheliasin; plasmic transmembrane protein X; transmembrane protease serine 2.

• Gene ID :

  50528

• Accession Number :

  NP_056590

• Reactivity :

  Mouse|Mus musculus

• Target :

  Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions. The spike proteins are synthesized and maintained in precursor intermediate folding states and proteolysis permits the refolding and energy release required to create stable virus-cell linkages and membrane coalescence. Facilitates human SARS coronavirus (SARS-CoV) infection via two independent mechanisms, proteolytic cleavage of ACE2, which might promote viral uptake, and cleavage of coronavirus spike glycoprotein which activates the glycoprotein for cathepsin L-independent host cell entry. Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV) . Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9) and is involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.

• Type :

  ELISA Kit

• Applications :

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method :

  Colorimetric

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane Protease, Serine 2 (TMPRSS2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transmembrane Protease, Serine 2 (TMPRSS2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transmembrane Protease, Serine 2 (TMPRSS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transmembrane Protease, Serine 2 (TMPRSS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Serum, plasma, tissue homogenates and other biological fluids.

• Detection Range :

  0.312-20ng/mL

• Recovery :

  Matrix Recovery Range (%) Average (%) serum (n=5) 82-89 85 EDTA plasma (n=5) 81-96 91 heparin plasma (n=5) 86-102 96 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 82-89 85 serum (n=5) 82-89 85 EDTA plasma (n=5) 81-96 91 EDTA plasma (n=5) 81-96 91 heparin plasma (n=5) 86-102 96 heparin plasma (n=5) 86-102 96

• Sensitivity :

  < 0.113 ng/mL

• Reconstitution :

  2°C to 8°C|-20°C

• Components :

  Component Amount Anti-Tmprss2 Microplate 96 Wells (12 x 8 Well Strips) Tmprss2 Lyophilized Standard 2 x 20 ng 100X Biotinylated Tmprss2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Tmprss2 Microplate 96 Wells (12 x 8 Well Strips) Anti-Tmprss2 Microplate 96 Wells (12 x 8 Well Strips) Tmprss2 Lyophilized Standard 2 x 20 ng Tmprss2 Lyophilized Standard 2 x 20 ng 100X Biotinylated Tmprss2 Detector Antibody 120 uL 100X Biotinylated Tmprss2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes :

  Formerly GWB-KBBTV7

• Specificity :

  This assay has high sensitivity and excellent specificity for detection of Transmembrane Protease, Serine 2 (TMPRSS2) . No significant cross-reactivity or interference between Transmembrane Protease, Serine 2 (TMPRSS2) and analogues was observed.

• Protein Length :

  490

• NCBI Gene Symbol :

  Tmprss2

• Protein Name :

  Transmembrane protease serine 2

• Gene Name URL :

  Tmprss2

• CAS Number :

  7732-18-5

• Nucleotide Accession Number :

  NM_015775.2