NR4A3 ELISA Kit (Rat)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Neuron Derived Orphan Receptor 1 (NOR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Neuron Derived Orphan Receptor 1 (NOR1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

7732-18-5

Nuclear receptor subfamily 4, group A, member 3

Neuron-derived orphan receptor 1; neuron-derived orphan receptor 1/2; NOR-2; nuclear hormone receptor NOR-1/NOR-2; nuclear receptor subfamily 4 group A member 3.

58853

NP_113816

Rat|Rattus norvegicus

Transcriptional activator that binds to regulatory elements in promoter regions in a cell- and response element (target) -specific manner (PubMed:7811288) . Induces gene expression by binding as monomers to the NR4A1 response element (NBRE) 5'-AAAAGGTCA-3' site and as homodimers to the Nur response element (NurRE) site in the promoter of their regulated target genes (PubMed:10523643) . Plays a role in the regulation of proliferation, survival and differentiation of many different cell types and also in metabolism and inflammation. Mediates proliferation of vascular smooth muscle, myeloid progenitor cell and type B pancreatic cells; promotes mitogen-induced vascular smooth muscle cell proliferation through transactivation of SKP2 promoter by binding a NBRE site (By similarity) . Upon PDGF stimulation, stimulates vascular smooth muscle cell proliferation by regulating CCND1 and CCND2 expression (PubMed:16945922) . In islets, induces type B pancreatic cell proliferation through up-regulation of genes that activate cell cycle, as well as genes that cause degradation of the CDKN1A (PubMed:24706823) . Negatively regulates myeloid progenitor cell proliferation by repressing RUNX1 in a NBRE site-independent manner. During inner ear, plays a role as a key mediator of the proliferative growth phase of semicircular canal development (By similarity) . Mediates also survival of neuron and smooth muscle cells; mediates CREB-induced neuronal survival, and during hippocampus development, plays a critical role in pyramidal cell survival and axonal guidance. Is required for S phase entry of the cell cycle and survival of smooth muscle cells by inducing CCND1, resulting in RB1 phosphorylation. Binds to NBRE motif in CCND1 promoter, resulting in the activation of the promoter and CCND1 transcription (By similarity) . Plays also a role in inflammation; Upon TNF stimulation, mediates monocyte adhesion by inducing the expression of VCAM1 and ICAM1 by binding to the NBRE consensus site (By similarity) . In mast cells activated by Fc-epsilon receptor cross-linking, promotes the synthesis and release of cytokines but impairs events leading to degranulation. Plays also a role in metabolism; by modulating feeding behavior; and by playing a role in energy balance by inhibiting the glucocorticoid-induced orexigenic neuropeptides AGRP expression, at least in part by forming a complex with activated NR3C1 on the AGRP-glucocorticoid response element (GRE), and thus weakening the DNA binding activity of NR3C1. Upon catecholamines stimulation, regulates gene expression that controls oxidative metabolism in skeletal muscle (By similarity) . Plays a role in glucose transport by regulating translocation of the SLC2A4 glucose transporter to the cell surface (By similarity) . Finally, during gastrulation plays a crucial role in the formation of anterior mesoderm by controlling cell migration (By similarity) . Also participates in cardiac hypertrophy by activating PARP1 (PubMed:25625556) .

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuron Derived Orphan Receptor 1 (NOR1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuron Derived Orphan Receptor 1 (NOR1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuron Derived Orphan Receptor 1 (NOR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neuron Derived Orphan Receptor 1 (NOR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Tissue homogenates and other biological fluids.

0.312-20ng/mL

< 0.122 ng/mL

2°C to 8°C|-20°C

Component Amount Anti-Nr4a3 Microplate 96 Wells (12 x 8 Well Strips) Nr4a3 Lyophilized Standard 2 x 20 ng 100X Biotinylated Nr4a3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Nr4a3 Microplate 96 Wells (12 x 8 Well Strips) Anti-Nr4a3 Microplate 96 Wells (12 x 8 Well Strips) Nr4a3 Lyophilized Standard 2 x 20 ng Nr4a3 Lyophilized Standard 2 x 20 ng 100X Biotinylated Nr4a3 Detector Antibody 120 uL 100X Biotinylated Nr4a3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

Formerly GWB-KBBSN6

This assay has high sensitivity and excellent specificity for detection of Neuron Derived Orphan Receptor 1 (NOR1) . No significant cross-reactivity or interference between Neuron Derived Orphan Receptor 1 (NOR1) and analogues was observed.

628

Nr4a3

Nuclear receptor subfamily 4 group A member 3

Nr4a3