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KLC1 ELISA Kit (Human)

CAT: 0247-OKCD00873-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD00873-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Kinesin Light Chain 1 (KLC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Kinesin Light Chain 1 (KLC1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Kinesin light chain 1
Gene Aliases
Kinesin 2 60/70kDa; kinesin light chain 1; KLC; KNS2; KNS2A; medulloblastoma antigen MU-MB-2.50.
Gene ID
3831
Accession Number
NP_001123579
Reactivity
Homo sapiens|Human
Target
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinesin Light Chain 1 (KLC1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kinesin Light Chain 1 (KLC1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kinesin Light Chain 1 (KLC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kinesin Light Chain 1 (KLC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
< 0.106 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-KLC1 Microplate 96 Wells (12 x 8 Well Strips) KLC1 Lyophilized Standard 2 x 20 ng 100X Biotinylated KLC1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-KLC1 Microplate 96 Wells (12 x 8 Well Strips) Anti-KLC1 Microplate 96 Wells (12 x 8 Well Strips) KLC1 Lyophilized Standard 2 x 20 ng KLC1 Lyophilized Standard 2 x 20 ng 100X Biotinylated KLC1 Detector Antibody 120 uL 100X Biotinylated KLC1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBDQ1
Specificity
This assay has high sensitivity and excellent specificity for detection of Kinesin 2 (KNS2) . No significant cross-reactivity or interference between Kinesin 2 (KNS2) and analogues was observed.
Protein Length
573
NCBI Gene Symbol
KLC1
Protein Name
Kinesin light chain 1
Gene Name URL
KLC1
Nucleotide Accession Number
NM_001130107.1

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