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CLIC1 ELISA Kit (Human)

CAT: 0247-OKCD00868-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD00868-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Chloride Intracellular Channel Protein 1 (CLIC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Chloride Intracellular Channel Protein 1 (CLIC1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Chloride intracellular channel 1
Gene Aliases
Chloride channel ABP; chloride intracellular channel protein 1; CL1C1; CLCNL1; G6; hRNCC; NCC27; nuclear chloride ion channel 27; nuclear chloride ion channel protein; p64CLCP; regulatory nuclear chloride ion channel protein; RNCC protein.
Gene ID
1192
Accession Number
NP_001274522 ÂÂ
Reactivity
Homo sapiens|Human
Target
Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chloride Intracellular Channel Protein 1 (CLIC1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chloride Intracellular Channel Protein 1 (CLIC1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chloride Intracellular Channel Protein 1 (CLIC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chloride Intracellular Channel Protein 1 (CLIC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
< 0.112 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount _x005F Anti-CLIC1 Microplate 96 Wells (12 x 8 Well Strips) _x005F CLIC1 Lyophilized Standard 2 x 20 ng _x005F 100X Biotinylated CLIC1 Detector Antibody 120 uL _x005F Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount Component Amount Anti-CLIC1 Microplate 96 Wells (12 x 8 Well Strips) Anti-CLIC1 Microplate 96 Wells (12 x 8 Well Strips) CLIC1 Lyophilized Standard 2 x 20 ng CLIC1 Lyophilized Standard 2 x 20 ng 100X Biotinylated CLIC1 Detector Antibody 120 uL 100X Biotinylated CLIC1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBCY1
Specificity
This assay has high sensitivity and excellent specificity for detection of Chloride Intracellular Channel Protein 1 (CLIC1) ._x005F No significant cross-reactivity or interference between Chloride Intracellular Channel Protein 1 (CLIC1) and analogues was observed.
Protein Length
241
NCBI Gene Symbol
CLIC1
Protein Name
Chloride intracellular channel protein 1
Gene Name URL
CLIC1
Nucleotide Accession Number
NM_001287593.1

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