COL5A2 ELISA Kit (Human)

CAT: 0247-OKCD00862-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD00862-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Collagen Type V Alpha 2 (COL5a2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Collagen Type V Alpha 2 (COL5a2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Collagen type V alpha 2 chain
Gene Aliases
AB collagen; collagen alpha-2 (V) chain; collagen, fetal membrane, A polypeptide; EDSC; EDSCL2; type V preprocollagen alpha 2 chain.
Gene ID
1290
Accession Number
NP_000384
Reactivity
Homo sapiens|Human
Target
Type V collagen is a member of group I collagen (fibrillar forming collagen) . It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Type V collagen is a key determinant in the assembly of tissue-specific matrices (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type V Alpha 2 (COL5a2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type V Alpha 2 (COL5a2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type V Alpha 2 (COL5a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Collagen Type V Alpha 2 (COL5a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.312-20ng/mL
Recovery
Matrix Recovery Range (%) Average (%) serum (n=5) 86-103 92 EDTA plasma (n=5) 80-94 88 heparin plasma (n=5) 83-94 86 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 86-103 92 serum (n=5) 86-103 92 EDTA plasma (n=5) 80-94 88 EDTA plasma (n=5) 80-94 88 heparin plasma (n=5) 83-94 86 heparin plasma (n=5) 83-94 86
Sensitivity
< 0.126 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-COL5A2 Microplate 96 Wells (12 x 8 Well Strips) COL5A2 Lyophilized Standard 2 x 20 ng 100X Biotinylated COL5A2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-COL5A2 Microplate 96 Wells (12 x 8 Well Strips) Anti-COL5A2 Microplate 96 Wells (12 x 8 Well Strips) COL5A2 Lyophilized Standard 2 x 20 ng COL5A2 Lyophilized Standard 2 x 20 ng 100X Biotinylated COL5A2 Detector Antibody 120 uL 100X Biotinylated COL5A2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBUT4
Specificity
This assay has high sensitivity and excellent specificity for detection of Collagen Type V Alpha 2 (COL5a2) . No significant cross-reactivity or interference between Collagen Type V Alpha 2 (COL5a2) and analogues was observed.
Protein Length
1499
NCBI Gene Symbol
COL5A2
Protein Name
Collagen alpha-2 (V) chain
Gene Name URL
COL5A2
Nucleotide Accession Number
NM_000393.3

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