GSTA2 ELISA Kit (Human)

• Reproducibility :

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Glutathione S Transferase Alpha 2 (GSTa2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Glutathione S Transferase Alpha 2 (GSTa2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name :

  Glutathione S-transferase alpha 2

• Gene Aliases :

  Glutathione S-alkyltransferase A2; glutathione S-aralkyltransferase A2; glutathione S-aryltransferase A2; glutathione S-transferase A2; GST class-alpha member 2; GST HA subunit 2; GST2; GSTA2-2; GST-gamma; GTA2; GTH2; liver GST2; S- (hydroxyalkyl) glutathione lyase A2; testis tissue sperm-binding protein Li 59n.

• Gene ID :

  2939

• Accession Number :

  NP_000837

• Reactivity :

  Homo sapiens|Human

• Target :

  Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

• Type :

  ELISA Kit

• Applications :

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method :

  Colorimetric

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Alpha 2 (GSTa2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Alpha 2 (GSTa2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Alpha 2 (GSTa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutathione S Transferase Alpha 2 (GSTa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range :

  0.312-20ng/mL

• Recovery :

  Matrix Recovery Range (%) Average (%) serum (n=5) 90-97 93 EDTA plasma (n=5) 78-102 91 heparin plasma (n=5) 78-96 82 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 90-97 93 serum (n=5) 90-97 93 EDTA plasma (n=5) 78-102 91 EDTA plasma (n=5) 78-102 91 heparin plasma (n=5) 78-96 82 heparin plasma (n=5) 78-96 82

• Sensitivity :

  < 0.125 ng/mL

• Reconstitution :

  2°C to 8°C|-20°C

• Components :

  Component Amount Anti-GSTA2 Microplate 96 Wells (12 x 8 Well Strips) GSTA2 Lyophilized Standard 2 x 20 ng 100X Biotinylated GSTA2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-GSTA2 Microplate 96 Wells (12 x 8 Well Strips) Anti-GSTA2 Microplate 96 Wells (12 x 8 Well Strips) GSTA2 Lyophilized Standard 2 x 20 ng GSTA2 Lyophilized Standard 2 x 20 ng 100X Biotinylated GSTA2 Detector Antibody 120 uL 100X Biotinylated GSTA2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes :

  Formerly GWB-KBBMM8

• Specificity :

  This assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Alpha 2 (GSTa2) . No significant cross-reactivity or interference between Glutathione S Transferase Alpha 2 (GSTa2) and analogues was observed.

• Protein Length :

  222

• NCBI Gene Symbol :

  GSTA2

• Protein Name :

  Glutathione S-transferase A2

• Gene Name URL :

  GSTA2

• CAS Number :

  7732-18-5

• Nucleotide Accession Number :

  NM_000846.4