IL1RAP ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Interleukin 1 Receptor Accessory Protein (IL1RAP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Interleukin 1 Receptor Accessory Protein (IL1RAP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Interleukin 1 receptor accessory protein

• Gene Aliases:

  6430709H04Rik; AI255955; AV239853; IL-; IL-1 receptor accessory protein; IL-1RAcP; interleukin-1 receptor accessory protein; interleukin-33 receptot beta chain.

• Gene ID:

  16180

• Accession Number:

  NP_001152790

• Reactivity:

  Mouse|Mus musculus

• Target:

  Coreceptor for IL1RL2 in the IL-36 signaling system. Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Coreceptor for IL1RL1 in the IL-33 signaling system. Can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD (PubMed:25908590) . May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (By similarity) .

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Accessory Protein (IL1RAP) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Accessory Protein (IL1RAP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Accessory Protein (IL1RAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 1 Receptor Accessory Protein (IL1RAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates and other biological fluids

• Detection Range:

  31.2-2,000pg/mL

• Recovery:

  Matrix Recovery Range (%) Average (%) serum (n=5) 84-92 89 EDTA plasma (n=5) 85-99 90 heparin plasma (n=5) 81-103 97 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 84-92 89 serum (n=5) 84-92 89 EDTA plasma (n=5) 85-99 90 EDTA plasma (n=5) 85-99 90 heparin plasma (n=5) 81-103 97 heparin plasma (n=5) 81-103 97

• Sensitivity:

  < 13.4 pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-Il1rap Microplate 96 Wells (12 x 8 Well Strips) Il1rap Lyophilized Standard 2 x 2 ng 100X Biotinylated Il1rap Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Il1rap Microplate 96 Wells (12 x 8 Well Strips) Anti-Il1rap Microplate 96 Wells (12 x 8 Well Strips) Il1rap Lyophilized Standard 2 x 2 ng Il1rap Lyophilized Standard 2 x 2 ng 100X Biotinylated Il1rap Detector Antibody 120 uL 100X Biotinylated Il1rap Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes:

  Formerly GWB-KBBQC3

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Accessory Protein (IL1RAP) . No significant cross-reactivity or interference between Interleukin 1 Receptor Accessory Protein (IL1RAP) and analogues was observed.

• Protein Length:

  570

• NCBI Gene Symbol:

  Il1rap

• Protein Name:

  Interleukin-1 receptor accessory protein

• Gene Name URL:

  Il1rap

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001159318.1