JAM2 ELISA Kit (Human)

CAT: 0247-OKCD00755-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD00755-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Junctional Adhesion Molecule 2 (JAM2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Junctional Adhesion Molecule 2 (JAM2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Junctional adhesion molecule 2
Gene Aliases
C21orf43; CD322; IBGC8; JAM-2; JAMB; JAM-B; JAM-IT/VE-JAM; Junctional adhesion molecule 2; junctional adhesion molecule B; PRO245; vascular endothelial junction-associated molecule; VEJAM; VE-JAM.
Gene ID
58494
Accession Number
NP_001257336
Reactivity
Homo sapiens|Human
Target
May play a role in the processes of lymphocyte homing to secondary lymphoid organs.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Junctional Adhesion Molecule 2 (JAM2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Junctional Adhesion Molecule 2 (JAM2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Junctional Adhesion Molecule 2 (JAM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Junctional Adhesion Molecule 2 (JAM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
31.25-2000pg/mL
Recovery
Matrix Recovery Range (%) Average (%) serum (n=5) 93-101 97 EDTA plasma (n=5) 80-101 92 heparin plasma (n=5) 80-99 90 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 93-101 97 serum (n=5) 93-101 97 EDTA plasma (n=5) 80-101 92 EDTA plasma (n=5) 80-101 92 heparin plasma (n=5) 80-99 90 heparin plasma (n=5) 80-99 90
Sensitivity
< 12.7 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-JAM2 Microplate 96 Wells (12 x 8 Well Strips) JAM2 Lyophilized Standard 2 x 2 ng 100X Biotinylated JAM2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-JAM2 Microplate 96 Wells (12 x 8 Well Strips) Anti-JAM2 Microplate 96 Wells (12 x 8 Well Strips) JAM2 Lyophilized Standard 2 x 2 ng JAM2 Lyophilized Standard 2 x 2 ng 100X Biotinylated JAM2 Detector Antibody 120 uL 100X Biotinylated JAM2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBKE3
Specificity
This assay has high sensitivity and excellent specificity for detection of Junctional Adhesion Molecule 2 (JAM2) . No significant cross-reactivity or interference between Junctional Adhesion Molecule 2 (JAM2) and analogues was observed.
Protein Length
298
NCBI Gene Symbol
JAM2
Protein Name
Junctional adhesion molecule B
Gene Name URL
JAM2
Nucleotide Accession Number
NM_001270407.1

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