GPAM ELISA Kit (Human)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Glycerol-3-phosphate acyltransferase, mitochondrial

• Gene Aliases:

  Glycerol-3-phosphate acyltransferase 1, mitochondrial; GPAT; GPAT1; GPAT-1.

• Gene ID:

  57678

• Accession Number:

  NP_001231878

• Reactivity:

  Homo sapiens|Human

• Target:

  Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Tissue homogenates, cell lysates and other biological fluids

• Detection Range:

  1.56-100ng/mL

• Recovery:

  Matrix Recovery Range (%) Average (%) serum (n=5) 93-101 97 EDTA plasma (n=5) 80-97 83 heparin plasma (n=5) 88-96 92 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 93-101 97 serum (n=5) 93-101 97 EDTA plasma (n=5) 80-97 83 EDTA plasma (n=5) 80-97 83 heparin plasma (n=5) 88-96 92 heparin plasma (n=5) 88-96 92

• Sensitivity:

  < 0.55 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-GPAM Microplate 96 Wells (12 x 8 Well Strips) GPAM Lyophilized Standard 2 x 100 ng 100X Biotinylated GPAM Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-GPAM Microplate 96 Wells (12 x 8 Well Strips) Anti-GPAM Microplate 96 Wells (12 x 8 Well Strips) GPAM Lyophilized Standard 2 x 100 ng GPAM Lyophilized Standard 2 x 100 ng 100X Biotinylated GPAM Detector Antibody 120 uL 100X Biotinylated GPAM Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes:

  Formerly GWB-KBBUO3

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) . No significant cross-reactivity or interference between Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) and analogues was observed.

• Protein Length:

  828

• NCBI Gene Symbol:

  GPAM

• Protein Name:

  Glycerol-3-phosphate acyltransferase 1, mitochondrial

• Gene Name URL:

  GPAM

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001244949.1