MGEA5 ELISA Kit (Rat)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Meningioma Expressed Antigen 5 (MGEA5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Meningioma Expressed Antigen 5 (MGEA5) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  O-GlcNAcase

• Gene Aliases:

  Beta-hexosaminidase; beta-N-acetylhexosaminidase; bifunctional protein NCOAT; meningioma expressed antigen 5 (hyaluronidase) ; meningioma-expressed antigen 5; Mgea5; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; Ncoat; nuclear cytoplasmic O-GlcNAcase and acetyltransferase; protein O-GlcNAcase; protein O-GlcNAcase-like.

• Gene ID:

  154968

• Accession Number:

  NP_571979

• Reactivity:

  Rat|Rattus norvegicus

• Target:

  Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:8034696) . Does not bind acetyl-CoA and does not have histone acetyltransferase activity.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Meningioma Expressed Antigen 5 (MGEA5) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Meningioma Expressed Antigen 5 (MGEA5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Meningioma Expressed Antigen 5 (MGEA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Meningioma Expressed Antigen 5 (MGEA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Urine

• Detection Range:

  1.56-100ng/mL

• Sensitivity:

  < 0.55 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-Mgea5 Microplate 96 Wells (12 x 8 Well Strips) Mgea5 Lyophilized Standard 2 x 100 ng 100X Biotinylated Mgea5 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Mgea5 Microplate 96 Wells (12 x 8 Well Strips) Anti-Mgea5 Microplate 96 Wells (12 x 8 Well Strips) Mgea5 Lyophilized Standard 2 x 100 ng Mgea5 Lyophilized Standard 2 x 100 ng 100X Biotinylated Mgea5 Detector Antibody 120 uL 100X Biotinylated Mgea5 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes:

  Formerly GWB-KBBDW9

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of N-Acetyl Beta-D-Glucosaminidase (NAGase) . No significant cross-reactivity or interference between N-Acetyl Beta-D-Glucosaminidase (NAGase) and analogues was observed.

• Protein Length:

  916

• NCBI Gene Symbol:

  Oga

• Protein Name:

  Protein O-GlcNAcase

• Gene Name URL:

  Oga

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_131904.1