CTBS ELISA Kit (Human)

CAT: 0247-OKCD00650-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD00650-96WSize:96 Well
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Di-N-Acetyl Chitobiase (CTBS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Di-N-Acetyl Chitobiase (CTBS) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Chitobiase
Gene Aliases
Chitobiase, di-N-acetyl-; CTB; di-N-acetylchitobiase.
Gene ID
1486
Accession Number
NP_004379
Reactivity
Homo sapiens|Human
Target
Involved in the degradation of asparagine-linked glycoproteins. Hydrolyze of N-acetyl-beta-D-glucosamine (1-4) N-acetylglucosamine chitobiose core from the reducing end of the bond, it requires prior cleavage by glycosylasparaginase.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Di-N-Acetyl Chitobiase (CTBS) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Di-N-Acetyl Chitobiase (CTBS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Di-N-Acetyl Chitobiase (CTBS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Di-N-Acetyl Chitobiase (CTBS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.071 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-CTBS Microplate 96 Wells (12 x 8 Well Strips) CTBS Lyophilized Standard 2 x 10 ng 100X Biotinylated CTBS Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-CTBS Microplate 96 Wells (12 x 8 Well Strips) Anti-CTBS Microplate 96 Wells (12 x 8 Well Strips) CTBS Lyophilized Standard 2 x 10 ng CTBS Lyophilized Standard 2 x 10 ng 100X Biotinylated CTBS Detector Antibody 120 uL 100X Biotinylated CTBS Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBDF2
Specificity
This assay has high sensitivity and excellent specificity for detection of Di-N-Acetyl Chitobiase (CTBS) . No significant cross-reactivity or interference between Di-N-Acetyl Chitobiase (CTBS) and analogues was observed.
Protein Length
385
NCBI Gene Symbol
CTBS
Protein Name
Di-N-acetylchitobiase
Gene Name URL
CTBS
Nucleotide Accession Number
NM_004388.2

Related Products

CatalogName
OKCA01029CTBS ELISA Kit (Bovine) (OKCA01029)
OACD02805-1MLCTBS Antibody
OACD02805-100ULCTBS Antibody
OCOA13128-20UGCTBS Protein Lysate
OPCD02493-10UGCTBS Recombinant Protein
OPCD02493-200UGCTBS Recombinant Protein
OPCD02493-50UGCTBS Recombinant Protein
ARP63779_P050-25ULCTBS Antibody - C-terminal region
AAP63779-100UGCTBS Peptide - C-terminal region (AAP63779)
ARP63779_P050CTBS antibody - C-terminal region (ARP63779_P050)

Alternative Products

CatalogName
E011841Human CTBS ELISA Kit
NBP3-27462Human Chitobiase/CTBS ELISA Kit (Colorimetric)
C8842Di-N-acetylchitobiase (CTBS) Human ELISA Kit
ELI-32160hHuman Di-N-acetylchitobiase,CTBS ELISA KIT
MBS458631-01Human Di-N-Acetyl Chitobiase (CTBS) ELISA Kit
MBS8803184-01Human CTBS (Di-N-Acetyl Chitobiase) ELISA Kit
KBH9971GENLISA Human Di-N-Acetyl Chitobiase (CTBS) ELISA
NBP3-31421Human Chitobiase/CTBS - Ready-To-Use ELISA Kit (Colorimetric)
C8840Di-N-Acetyl Chitobiase (CTBS) Human ELISA Kit
KBH20590GENLISA™ Human Di - N - Acetyl Chitobiase (CTBS) ELISA

Popular Products