TRA2B ELISA Kit (Human)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Transformer 2 Beta (TRA2b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Transformer 2 Beta (TRA2b) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Transformer 2 beta homolog

• Gene Aliases:

  Htra2-beta; PPP1R156; protein phosphatase 1, regulatory subunit 156; SFRS10; Splicing factor, arginine/serine-rich 10; splicing factor, arginine/serine-rich 10 (transformer 2 homolog, Drosophila) ; SRFS10; TRA-2 beta; TRA2-BETA; TRAN2B; transformer-2 protein homolog B; transformer-2 protein homolog beta.

• Gene ID:

  6434

• Accession Number:

  NP_001230808

• Reactivity:

  Homo sapiens|Human

• Target:

  Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transformer 2 Beta (TRA2b) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transformer 2 Beta (TRA2b) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transformer 2 Beta (TRA2b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transformer 2 Beta (TRA2b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Tissue homogenates, cell lysates and other biological fluids

• Detection Range:

  0.156-10ng/mL

• Sensitivity:

  < 0.057 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-TRA2B Microplate 96 Wells (12 x 8 Well Strips) TRA2B Lyophilized Standard 2 x 10 ng 100X Biotinylated TRA2B Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-TRA2B Microplate 96 Wells (12 x 8 Well Strips) Anti-TRA2B Microplate 96 Wells (12 x 8 Well Strips) TRA2B Lyophilized Standard 2 x 10 ng TRA2B Lyophilized Standard 2 x 10 ng 100X Biotinylated TRA2B Detector Antibody 120 uL 100X Biotinylated TRA2B Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes:

  Formerly GWB-KBBYU4

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Transformer 2 Beta (TRA2b) . No significant cross-reactivity or interference between Transformer 2 Beta (TRA2b) and analogues was observed.

• Protein Length:

  288

• NCBI Gene Symbol:

  TRA2B

• Protein Name:

  Transformer-2 protein homolog beta

• Gene Name URL:

  TRA2B

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001243879.1