Products for Research Use Only
RegisterLogin

ARPC2 ELISA Kit (Human)

CAT: 0247-OKCD00572-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD00572-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Actin related protein 2/3 complex subunit 2
Gene Aliases
Actin related protein 2/3 complex, subunit 2, 34kDa; actin-related protein 2/3 complex subunit 2; ARC34; arp2/3 complex 34 kDa subunit; ARP2/3 protein complex subunit 34; p34-Arc; PNAS-139; PRO2446; testis tissue sperm-binding protein Li 53e.
Gene ID
10109
Accession Number
NP_005722
Reactivity
Homo sapiens|Human
Target
Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Related Protein 2/3 Complex Subunit 2 (ARPC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.068 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-ARPC2 Microplate 96 Wells (12 x 8 Well Strips) ARPC2 Lyophilized Standard 2 x 10 ng 100X Biotinylated ARPC2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-ARPC2 Microplate 96 Wells (12 x 8 Well Strips) Anti-ARPC2 Microplate 96 Wells (12 x 8 Well Strips) ARPC2 Lyophilized Standard 2 x 10 ng ARPC2 Lyophilized Standard 2 x 10 ng 100X Biotinylated ARPC2 Detector Antibody 120 uL 100X Biotinylated ARPC2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBXP7
Specificity
This assay has high sensitivity and excellent specificity for detection of Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) . No significant cross-reactivity or interference between Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) and analogues was observed.
Protein Length
300
NCBI Gene Symbol
ARPC2
Protein Name
Actin-related protein 2/3 complex subunit 2
Gene Name URL
ARPC2
Nucleotide Accession Number
NM_005731.3

Related Products

CatalogName
OAGA00146-100ULARPC2 antibody
OPCD01486-50UGARPC2 Recombinant Protein
OPCD01486-200UGARPC2 Recombinant Protein
OCOA05507-20UGARPC2 Protein Lysate
OCOA12467-20UGARPC2 Protein Lysate
OPCD01486-10UGARPC2 Recombinant Protein
ARP90207_P050Arpc2 Antibody Middle region
ARP90207_P050-25ULARPC2 Antibody - middle region
OOCA00196-25TOOCA00196-25T - Human ARPC2 Primer Pair 2
OOCA00197-25TOOCA00197-25T - Human ARPC2 Primer Pair 3

Alternative Products

CatalogName
GTR18823732-01Human ARPC2 ELISA Kit
NBP3-27231Human ARPC2 ELISA Kit (Colorimetric)
GTR18828124-01Human ARPC2 ELISA Kit (Ready to Use)
NBP3-31038Human ARPC2 - Ready-To-Use ELISA Kit (Colorimetric)
MBS2706730-01Human Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) ELISA Kit
CSB-EL002127HU-01Human Actin-related protein 2/3 complex subunit 2 (ARPC2) ELISA kit
ELK4658-01Human ARPC2 (Actin Related Protein 2/3 Complex Subunit 2) ELISA Kit
KBH20185GENLISA™ Human Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) ELISA
B2359Actin-Related protein 2/3 complex subunit 2 (ARPC2) Human ELISA Kit
B2277Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) Human ELISA Kit

Popular Products