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PNMA2 ELISA Kit (Human)

CAT: 0247-OKCD00534-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD00534-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Paraneoplastic Antigen MA2 (PNMA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Paraneoplastic Antigen MA2 (PNMA2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
PNMA family member 2
Gene Aliases
40 kDa neuronal protein; MA2; MM2; onconeuronal antigen MA2; paraneoplastic antigen Ma2; paraneoplastic Ma antigen 2; paraneoplastic neuronal antigen MM2; retrotransposon gag domain containing 2; RGAG2.
Gene ID
10687
Accession Number
NP_009188
Reactivity
Homo sapiens|Human
Target
Paraneoplastic antigen Ma2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paraneoplastic Antigen MA2 (PNMA2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paraneoplastic Antigen MA2 (PNMA2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paraneoplastic Antigen MA2 (PNMA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Paraneoplastic Antigen MA2 (PNMA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.058 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-PNMA2 Microplate 96 Wells (12 x 8 Well Strips) PNMA2 Lyophilized Standard 2 x 10 ng 100X Biotinylated PNMA2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-PNMA2 Microplate 96 Wells (12 x 8 Well Strips) Anti-PNMA2 Microplate 96 Wells (12 x 8 Well Strips) PNMA2 Lyophilized Standard 2 x 10 ng PNMA2 Lyophilized Standard 2 x 10 ng 100X Biotinylated PNMA2 Detector Antibody 120 uL 100X Biotinylated PNMA2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBBX8
Specificity
This assay has high sensitivity and excellent specificity for detection of Paraneoplastic Antigen MA2 (PNMA2) . No significant cross-reactivity or interference between Paraneoplastic Antigen MA2 (PNMA2) and analogues was observed.
Protein Length
364
NCBI Gene Symbol
PNMA2
Protein Name
Paraneoplastic antigen Ma2
Gene Name URL
PNMA2
Nucleotide Accession Number
NM_007257.5

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