Products for Research Use Only
RegisterLogin

SNAPIN ELISA Kit (Human)

CAT: 0247-OKCD00523-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD00523-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level SNAP Associated Protein (SNAPAP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level SNAP Associated Protein (SNAPAP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
SNAP associated protein
Gene Aliases
Biogenesis of lysosomal organelles complex-1, subunit 7; biogenesis of lysosome-related organelles complex 1 subunit 7; BLOC-1 related complex subunit 3; BLOC-1 subunit 7; BLOC1S7; BLOS7; BORCS3; SNAP-25-binding protein; SNAPAP; SNARE associated protein snapin; SNARE-associated protein Snapin; synaptosomal-associated protein 25-binding protein.
Gene ID
23557
Accession Number
NP_036569
Reactivity
Homo sapiens|Human
Target
Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking and synaptic vesicle recycling. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells (PubMed:17182842, PubMed:18167355) . As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor (PubMed:25898167) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SNAP Associated Protein (SNAPAP) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SNAP Associated Protein (SNAPAP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SNAP Associated Protein (SNAPAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SNAP Associated Protein (SNAPAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.066 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-SNAPIN Microplate 96 Wells (12 x 8 Well Strips) SNAPIN Lyophilized Standard 2 x 10 ng 100X Biotinylated SNAPIN Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-SNAPIN Microplate 96 Wells (12 x 8 Well Strips) Anti-SNAPIN Microplate 96 Wells (12 x 8 Well Strips) SNAPIN Lyophilized Standard 2 x 10 ng SNAPIN Lyophilized Standard 2 x 10 ng 100X Biotinylated SNAPIN Detector Antibody 120 uL 100X Biotinylated SNAPIN Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBPD8
Specificity
This assay has high sensitivity and excellent specificity for detection of SNAP Associated Protein (SNAPAP) . No significant cross-reactivity or interference between SNAP Associated Protein (SNAPAP) and analogues was observed.
Protein Length
136
NCBI Gene Symbol
SNAPIN
Protein Name
SNARE-associated protein Snapin
Gene Name URL
SNAPIN
Nucleotide Accession Number
NM_012437.5

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

Related Products

CatalogName
OACD00958-100ULSNAPIN Antibody
OAAN04207-100ULSNAPIN Antibody
ARP80039_P050Anti-SNAPIN
OPPA01848-10UGSNAPIN Protein
OACD00958-1MLSNAPIN Antibody
OAAN04207-200ULSNAPIN Antibody
OAAN04207-50ULSNAPIN Antibody
OCOA11485-20UGSNAPIN Protein Lysate
OPCD00646-10UGSNAPIN Recombinant Protein
ARP88211_P050SNAPIN Antibody (ARP88211_P050)

Alternative Products

CatalogName
KBH6186GENLISA™ Human SNARE-associated Protein Snapin (SNAPIN) ELISA
ELI-18155hHuman SNARE-associated protein Snapin, SNAPIN ELISA KIT
CSB-EL021882HU-01Human SNARE-associated protein Snapin (SNAPIN) ELISA kit
EF003090Human SNAPIN ELISA KIT
H1347SNARE-associated protein Snapin (SNAPIN) Human ELISA Kit
JOT-EK6186Hu-01Human SNARE-associated protein Snapin,SNAPIN ELISA KIT
OM306135-01SNAPIN Human
MBS9327038-01Mouse SNARE-associated protein Snapin, SNAPIN ELISA Kit
H1348SNARE-associated protein Snapin (SNAPIN) Mouse ELISA Kit
MBS1610989-01Rat SNARE-associated protein Snapin, Snapin ELISA KIT

Popular Products