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STAR ELISA Kit (Human)

CAT: 0247-OKCD00508-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD00508-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Steroidogenic Acute Regulatory Protein (STAR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Steroidogenic Acute Regulatory Protein (STAR) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Steroidogenic acute regulatory protein
Gene Aliases
Cholesterol trafficker; mitochondrial steroid acute regulatory protein; StAR related lipid transfer (START) domain containing 1; STARD1; START domain containing 1; START domain-containing protein 1; steroid acute regulatory protein; steroidogenic acute regulator; steroidogenic acute regulatory protein, mitochondrial; testis secretory sperm-binding protein Li 241mP.
Gene ID
6770
Accession Number
NP_000340
Reactivity
Homo sapiens|Human
Target
Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Steroidogenic Acute Regulatory Protein (STAR) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Steroidogenic Acute Regulatory Protein (STAR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Steroidogenic Acute Regulatory Protein (STAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Steroidogenic Acute Regulatory Protein (STAR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.064 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-STAR Microplate 96 Wells (12 x 8 Well Strips) STAR Lyophilized Standard 2 x 10 ng 100X Biotinylated STAR Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-STAR Microplate 96 Wells (12 x 8 Well Strips) Anti-STAR Microplate 96 Wells (12 x 8 Well Strips) STAR Lyophilized Standard 2 x 10 ng STAR Lyophilized Standard 2 x 10 ng 100X Biotinylated STAR Detector Antibody 120 uL 100X Biotinylated STAR Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBPK9
Specificity
This assay has high sensitivity and excellent specificity for detection of Steroidogenic Acute Regulatory Protein (STAR) . No significant cross-reactivity or interference between Steroidogenic Acute Regulatory Protein (STAR) and analogues was observed.
Protein Length
285
NCBI Gene Symbol
STAR
Protein Name
Steroidogenic acute regulatory protein, mitochondrial
Gene Name URL
STAR
Nucleotide Accession Number
NM_000349.2

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