CELSR2 ELISA Kit (Human)

CAT: 0247-OKCD00504-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD00504-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Cadherin EGF LAG seven-pass G-type receptor 2
Gene Aliases
ADGRC2; adhesion G protein-coupled receptor C2; cadherin EGF LAG seven-pass G-type receptor 2; cadherin family member 10; cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila) ; CDHF10; EGFL2; EGF-like protein 2; EGF-like-domain, multiple 2; epidermal growth factor-like 2; epidermal growth factor-like protein 2; flamingo homolog 3; Flamingo1; MEGF3; multiple EGF-like domains protein 3; multiple epidermal growth factor-like domains 3; multiple epidermal growth factor-like domains protein 3.
Gene ID
1952
Accession Number
NP_001399
Reactivity
Homo sapiens|Human
Target
Receptor that may have an important role in cell/cell signaling during nervous system formation.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.064 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-CELSR2 Microplate 96 Wells (12 x 8 Well Strips) CELSR2 Lyophilized Standard 2 x 10 ng 100X Biotinylated CELSR2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-CELSR2 Microplate 96 Wells (12 x 8 Well Strips) Anti-CELSR2 Microplate 96 Wells (12 x 8 Well Strips) CELSR2 Lyophilized Standard 2 x 10 ng CELSR2 Lyophilized Standard 2 x 10 ng 100X Biotinylated CELSR2 Detector Antibody 120 uL 100X Biotinylated CELSR2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBTK5
Specificity
This assay has high sensitivity and excellent specificity for detection of Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) . No significant cross-reactivity or interference between Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) and analogues was observed.
Protein Length
2923
NCBI Gene Symbol
CELSR2
Protein Name
Cadherin EGF LAG seven-pass G-type receptor 2
Gene Name URL
CELSR2
Nucleotide Accession Number
NM_001408.2

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