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ATP4A ELISA Kit (Mouse)

CAT: 0247-OKCD00502-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD00502-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
ATPase, H+/K+ exchanging, gastric, alpha polypeptide
Gene Aliases
ATPase, H+/K+ exchanging, alpha polypeptide; ATPase, H+/K+ transporting, alpha polypeptide; gastric H (+) /K (+) ATPase subunit alpha; H+/K+-ATPase alpha; H+K+-transporting alpha 1; potassium-transporting ATPase alpha chain 1; proton pump.
Gene ID
11944
Accession Number
NP_061201
Reactivity
Mouse|Mus musculus
Target
Sodium/potassium-transporting ATPase subunit alpha
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.055 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Atp4a Microplate 96 Wells (12 x 8 Well Strips) Atp4a Lyophilized Standard 2 x 10 ng 100X Biotinylated Atp4a Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Atp4a Microplate 96 Wells (12 x 8 Well Strips) Anti-Atp4a Microplate 96 Wells (12 x 8 Well Strips) Atp4a Lyophilized Standard 2 x 10 ng Atp4a Lyophilized Standard 2 x 10 ng 100X Biotinylated Atp4a Detector Antibody 120 uL 100X Biotinylated Atp4a Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBLN7
Specificity
This assay has high sensitivity and excellent specificity for detection of ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) . No significant cross-reactivity or interference between ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) and analogues was observed.
Protein Length
1025
NCBI Gene Symbol
Atp4a
Protein Name
Sodium/potassium-transporting ATPase subunit alpha
Gene Name URL
Atp4a
Nucleotide Accession Number
NM_018731.3

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