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PSME3 ELISA Kit (Human)

CAT: 0247-OKCD00498-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD00498-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Proteasome Activator Subunit 3 (PSME3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Proteasome Activator Subunit 3 (PSME3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Proteasome activator subunit 3
Gene Aliases
11S regulator complex gamma subunit;11S regulator complex subunit gamma; activator of multicatalytic protease subunit 3; epididymis secretory protein Li 283; HEL-S-283; Ki; Ki antigen; Ki nuclear autoantigen; PA28 gamma variant 5; PA28G; PA28gamma; PA28-gamma; proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) ; proteasome activator 28 subunit gamma; proteasome activator 28-gamma; proteasome activator complex subunit 3; REG gamma-3; REG-GAMMA.
Gene ID
10197
Accession Number
NP_005780
Reactivity
Homo sapiens|Human
Target
Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (PubMed:25361978) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteasome Activator Subunit 3 (PSME3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteasome Activator Subunit 3 (PSME3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteasome Activator Subunit 3 (PSME3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Proteasome Activator Subunit 3 (PSME3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.056 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-PSME3 Microplate 96 Wells (12 x 8 Well Strips) PSME3 Lyophilized Standard 2 x 10 ng 100X Biotinylated PSME3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-PSME3 Microplate 96 Wells (12 x 8 Well Strips) Anti-PSME3 Microplate 96 Wells (12 x 8 Well Strips) PSME3 Lyophilized Standard 2 x 10 ng PSME3 Lyophilized Standard 2 x 10 ng 100X Biotinylated PSME3 Detector Antibody 120 uL 100X Biotinylated PSME3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBDP8
Specificity
This assay has high sensitivity and excellent specificity for detection of Proteasome Activator Subunit 3 (PSME3) . No significant cross-reactivity or interference between Proteasome Activator Subunit 3 (PSME3) and analogues was observed.
Protein Length
254
NCBI Gene Symbol
PSME3
Protein Name
Proteasome activator complex subunit 3
Gene Name URL
PSME3
Nucleotide Accession Number
NM_005789.3

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