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CBR3 ELISA Kit (Human)

CAT: 0247-OKCD00458-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD00458-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Carbonyl Reductase 3 (CBR3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Carbonyl Reductase 3 (CBR3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Carbonyl reductase 3
Gene Aliases
Carbonyl reductase (NADPH) 3; carbonyl reductase [NADPH] 3; epididymis secretory protein Li 25; hCBR3; HEL-S-25; NADPH-dependent carbonyl reductase 3; SDR21C2; short chain dehydrogenase/reductase family 21C member 2.
Gene ID
874
Accession Number
NP_001227
Reactivity
Homo sapiens|Human
Target
Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonyl Reductase 3 (CBR3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonyl Reductase 3 (CBR3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonyl Reductase 3 (CBR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carbonyl Reductase 3 (CBR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.066 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-CBR3 Microplate 96 Wells (12 x 8 Well Strips) CBR3 Lyophilized Standard 2 x 10 ng 100X Biotinylated CBR3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-CBR3 Microplate 96 Wells (12 x 8 Well Strips) Anti-CBR3 Microplate 96 Wells (12 x 8 Well Strips) CBR3 Lyophilized Standard 2 x 10 ng CBR3 Lyophilized Standard 2 x 10 ng 100X Biotinylated CBR3 Detector Antibody 120 uL 100X Biotinylated CBR3 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBXY9
Specificity
This assay has high sensitivity and excellent specificity for detection of Carbonyl Reductase 3 (CBR3) . No significant cross-reactivity or interference between Carbonyl Reductase 3 (CBR3) and analogues was observed.
Protein Length
277
NCBI Gene Symbol
CBR3
Protein Name
Carbonyl reductase [NADPH] 3
Gene Name URL
CBR3
Nucleotide Accession Number
NM_001236.3

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