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GNA11 ELISA Kit (Human)

CAT: 0247-OKCD00454-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD00454-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level G Protein Alpha 11 (GNa11) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level G Protein Alpha 11 (GNa11) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
G protein subunit alpha 11
Gene Aliases
FBH; FBH2; FHH2; g alpha-11; GNA-11; guanine nucleotide binding protein (G protein), alpha 11 (Gq class) ; guanine nucleotide-binding protein G (y) subunit alpha; guanine nucleotide-binding protein subunit alpha-11; HHC2; HYPOC2.
Gene ID
2767
Accession Number
NP_002058
Reactivity
Homo sapiens|Human
Target
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Acts as an activator of phospholipase C.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Alpha 11 (GNa11) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Alpha 11 (GNa11) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Alpha 11 (GNa11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of G Protein Alpha 11 (GNa11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery Range (%) Average (%) 82-99 92 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) 82-99 92 82-99 92
Sensitivity
< 0.059 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-GNA11 Microplate 96 Wells (12 x 8 Well Strips) GNA11 Lyophilized Standard 2 x 10 ng 100X Biotinylated GNA11 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-GNA11 Microplate 96 Wells (12 x 8 Well Strips) Anti-GNA11 Microplate 96 Wells (12 x 8 Well Strips) GNA11 Lyophilized Standard 2 x 10 ng GNA11 Lyophilized Standard 2 x 10 ng 100X Biotinylated GNA11 Detector Antibody 120 uL 100X Biotinylated GNA11 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBQH8
Specificity
This assay has high sensitivity and excellent specificity for detection of G Protein Alpha 11 (GNa11) . No significant cross-reactivity or interference between G Protein Alpha 11 (GNa11) and analogues was observed.
Protein Length
359
NCBI Gene Symbol
GNA11
Protein Name
Guanine nucleotide-binding protein subunit alpha-11
Gene Name URL
GNA11
Nucleotide Accession Number
NM_002067.4

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