APRT ELISA Kit (Human)

CAT: 0247-OKCD00324-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD00324-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Adenine Phosphoribosyltransferase (APRT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Adenine Phosphoribosyltransferase (APRT) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Adenine phosphoribosyltransferase
Gene Aliases
Adenine phosphoribosyltransferase; AMP; AMP diphosphorylase; AMP pyrophosphorylase; APRTD; transphosphoribosidase.
Gene ID
353
Accession Number
NP_000476
Reactivity
Homo sapiens|Human
Target
Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenine Phosphoribosyltransferase (APRT) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenine Phosphoribosyltransferase (APRT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenine Phosphoribosyltransferase (APRT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adenine Phosphoribosyltransferase (APRT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.061 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-APRT Microplate 96 Wells (12 x 8 Well Strips) APRT Lyophilized Standard 2 x 10 ng 100X Biotinylated APRT Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-APRT Microplate 96 Wells (12 x 8 Well Strips) Anti-APRT Microplate 96 Wells (12 x 8 Well Strips) APRT Lyophilized Standard 2 x 10 ng APRT Lyophilized Standard 2 x 10 ng 100X Biotinylated APRT Detector Antibody 120 uL 100X Biotinylated APRT Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBEK1
Specificity
This assay has high sensitivity and excellent specificity for detection of Adenine Phosphoribosyltransferase (APRT) . No significant cross-reactivity or interference between Adenine Phosphoribosyltransferase (APRT) and analogues was observed.
Protein Length
180
NCBI Gene Symbol
APRT
Protein Name
Adenine phosphoribosyltransferase
Gene Name URL
APRT
Nucleotide Accession Number
NM_000485.2

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