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LY75 ELISA Kit (Mouse)

CAT: 0247-OKCD00285-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD00285-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Lymphocyte Antigen 75 (LY75) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Lymphocyte Antigen 75 (LY75) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Lymphocyte antigen 75
Gene Aliases
CD205; DEC205; DEC-205; ly-75; lymphocyte antigen 75.
Gene ID
17076
Accession Number
NP_038853
Reactivity
Mouse|Mus musculus
Target
Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment. Causes reduced proliferation of B lymphocytes (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Antigen 75 (LY75) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Antigen 75 (LY75) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Antigen 75 (LY75), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lymphocyte Antigen 75 (LY75) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell Lysates and other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
< 0.061 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Ly75 Microplate 96 Wells (12 x 8 Well Strips) Ly75 Lyophilized Standard 2 x 10 ng 100X Biotinylated Ly75 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Ly75 Microplate 96 Wells (12 x 8 Well Strips) Anti-Ly75 Microplate 96 Wells (12 x 8 Well Strips) Ly75 Lyophilized Standard 2 x 10 ng Ly75 Lyophilized Standard 2 x 10 ng 100X Biotinylated Ly75 Detector Antibody 120 uL 100X Biotinylated Ly75 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBYF3
Specificity
This assay has high sensitivity and excellent specificity for detection of Lymphocyte Antigen 75 (LY75) . No significant cross-reactivity or interference between Lymphocyte Antigen 75 (LY75) and analogues was observed.
Protein Length
1723
NCBI Gene Symbol
Ly75
Protein Name
Lymphocyte antigen 75
Gene Name URL
Ly75
Nucleotide Accession Number
NM_013825.3

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