PGLYRP1 ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Peptidoglycan Recognition Protein 1 (PGLYRP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Peptidoglycan Recognition Protein 1 (PGLYRP1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Peptidoglycan recognition protein 1

• Gene Aliases:

  Cytokine tag7; peptidoglycan recognition protein 1; Peptidoglycan recognition protein short; PG; Pglyrp; PGRP; PGRP-S; Tag; Tag7; Tas; Tasg7; Tnfs; Tnfsf3l.

• Gene ID:

  21946

• Accession Number:

  NP_033428

• Reactivity:

  Mouse|Mus musculus

• Target:

  Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram-negative bacteria. Involved in innate immunity. May function in intracellular killing of bacteria. The soluble form triggers apoptosis in vitro.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidoglycan Recognition Protein 1 (PGLYRP1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidoglycan Recognition Protein 1 (PGLYRP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidoglycan Recognition Protein 1 (PGLYRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peptidoglycan Recognition Protein 1 (PGLYRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma, tissue homogenates and other biological fluids.

• Detection Range:

  15.6-1,000pg/mL

• Recovery:

  Matrix Recovery Range (%) Average (%) serum (n=5) 98-105 102 EDTA plasma (n=5) 85-94 89 heparin plasma (n=5) 78-96 88 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 98-105 102 serum (n=5) 98-105 102 EDTA plasma (n=5) 85-94 89 EDTA plasma (n=5) 85-94 89 heparin plasma (n=5) 78-96 88 heparin plasma (n=5) 78-96 88

• Sensitivity:

  < 5.9 pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-Pglyrp1 Microplate 96 Wells (12 x 8 Well Strips) Pglyrp1 Lyophilized Standard 2 x 1 ng 100X Biotinylated Pglyrp1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Pglyrp1 Microplate 96 Wells (12 x 8 Well Strips) Anti-Pglyrp1 Microplate 96 Wells (12 x 8 Well Strips) Pglyrp1 Lyophilized Standard 2 x 1 ng Pglyrp1 Lyophilized Standard 2 x 1 ng 100X Biotinylated Pglyrp1 Detector Antibody 120 uL 100X Biotinylated Pglyrp1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes:

  Formerly GWB-KBBHO7

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Peptidoglycan Recognition Protein 1 (PGLYRP1) . No significant cross-reactivity or interference between Peptidoglycan Recognition Protein 1 (PGLYRP1) and analogues was observed.

• Protein Length:

  182

• NCBI Gene Symbol:

  Pglyrp1

• Protein Name:

  Peptidoglycan recognition protein 1

• Gene Name URL:

  Pglyrp1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_009402.2