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CXCL17 ELISA Kit (Rat)

CAT: 0247-OKCD00185-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD00185-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level VEGF Co Regulated Chemokine 1 (VCC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level VEGF Co Regulated Chemokine 1 (VCC1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
C-X-C motif chemokine ligand 17
Gene Aliases
Chemokine (C-X-C motif) ligand 17; C-X-C motif chemokine 17; RGD1304717; VEGF coregulated chemokine 1; VEGF co-regulated chemokine 1.
Gene ID
308436
Accession Number
NP_001100961
Reactivity
Rat|Rattus norvegicus
Target
C-X-C motif chemokine ligand 17
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGF Co Regulated Chemokine 1 (VCC1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VEGF Co Regulated Chemokine 1 (VCC1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VEGF Co Regulated Chemokine 1 (VCC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VEGF Co Regulated Chemokine 1 (VCC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids.
Detection Range
15.6-1,000pg/mL
Recovery
Matrix Recovery Range (%) Average (%) _x005F serum (n=5) 78-104 84 _x005F EDTA plasma (n=5) 79-105 92 _x005F heparin plasma (n=5) 80-97 86 _x005F Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 78-104 84 serum (n=5) 78-104 84 EDTA plasma (n=5) 79-105 92 EDTA plasma (n=5) 79-105 92 heparin plasma (n=5) 80-97 86 heparin plasma (n=5) 80-97 86
Sensitivity
< 6.1 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount _x005F Anti-Cxcl17 Microplate 96 Wells (12 x 8 Well Strips) _x005F Cxcl17 Lyophilized Standard 2 x 1 ng _x005F 100X Biotinylated Cxcl17 Detector Antibody 120 uL _x005F Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount Component Amount Anti-Cxcl17 Microplate 96 Wells (12 x 8 Well Strips) Anti-Cxcl17 Microplate 96 Wells (12 x 8 Well Strips) Cxcl17 Lyophilized Standard 2 x 1 ng Cxcl17 Lyophilized Standard 2 x 1 ng 100X Biotinylated Cxcl17 Detector Antibody 120 uL 100X Biotinylated Cxcl17 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBWY8
Specificity
This assay has high sensitivity and excellent specificity for detection of VEGF Co Regulated Chemokine 1 (VCC1) ._x005F No significant cross-reactivity or interference between VEGF Co Regulated Chemokine 1 (VCC1) and analogues was observed.
Protein Length
119
NCBI Gene Symbol
Cxcl17
Protein Name
C-X-C motif chemokine ligand 17
Gene Name URL
Cxcl17
Nucleotide Accession Number
NM_001107491.1

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

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