VIM ELISA Kit (Rat)

CAT: 0247-OKCA02611-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCA02611-96WSize:96 Well
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Aliases
RP11-124N14.1, FLJ36605
Gene ID
81818
Accession Number
NP_112402
Reactivity
Rat
Target
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Vim ELISA Kit (Rat) (OKCA02611) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Vim has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Vim is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Vim captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
23.5 - 1,500 ng/mL
Recovery
Sample Type Mean Recovery (%) Range (%) Serum (n=5) 102 95-107 EDTA Plasma (n=4) 90 85-94 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 102 95-107 Serum (n=5) 102 95-107 EDTA Plasma (n=4) 90 85-94 EDTA Plasma (n=4) 90 85-94
Sensitivity
5.8 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
VIM
Protein Name
Vimentin
Gene Name URL
VIM
Nucleotide Accession Number
NM_031140

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