TP53 ELISA Kit (Rat)

• Reproducibility:

  Mean Inter-Assay: CV

• Gene Aliases:

  FLJ92943, LFS1, TRP53, p53, p53 antigen, p53 transformation suppressor, p53 tumor suppressor, phosphoprotein p53, transformation-related protein 53

• Gene ID:

  100009292

• Accession Number:

  NP_001075873

• Reactivity:

  Rat

• Target:

  Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.

• Type:

  ELISA Kit

• Assay Principle:

  Aviva Systems Biology TP53 ELISA Kit (Rat) (OKCA02584) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TP53 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TP53 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard TP53 captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates

• Detection Range:

  12.5 - 800 pg/mL

• Recovery:

  Sample Type Mean Recovery (%) Range (%) Serum (n=5) 89 84-93 EDTA Plasma (n=4) 95 90-100 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 89 84-93 Serum (n=5) 89 84-93 EDTA Plasma (n=4) 95 90-100 EDTA Plasma (n=4) 95 90-100

• Sensitivity:

  3.12 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• NCBI Gene Symbol:

  TP53

• Protein Name:

  Cellular tumor antigen p53

• Gene Name URL:

  TP53

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001082404