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IL15 ELISA Kit (Pig)

CAT: 0247-OKCA02500-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA02500-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Aliases
IL-15
Gene ID
397683
Accession Number
NP_999555
Reactivity
Pig
Target
Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology IL15 ELISA Kit (Pig) (OKCA02500) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL15 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard IL15 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Culture Supernates, Tissue Homogenates, Cell Lysates
Detection Range
0.78 - 50 pg/mL
Recovery
Sample Type Mean Recovery (%) Range (%) Serum (n=5) 100 97-103 EDTA Plasma (n=4) 84 80-88 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 100 97-103 Serum (n=5) 100 97-103 EDTA Plasma (n=4) 84 80-88 EDTA Plasma (n=4) 84 80-88
Sensitivity
0.195 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
IL15
Protein Name
Interleukin-15
Gene Name URL
IL15
Nucleotide Accession Number
NM_214390

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