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PTGDS ELISA Kit (Mouse)

CAT: 0247-OKCA02434-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA02434-96WSize:96 Well
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Reproducibility
Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Aliases
L-P, PGD, Ptg, PGD2, PGDS, 21kDa, PGDS2, Ptgs3, L-PGDS
Gene ID
19215
Accession Number
NP_032989
Reactivity
Mouse
Target
Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Ptgds ELISA Kit (Mouse) (OKCA02434) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ptgds has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ptgds is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Ptgds captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
4.7 - 300 ng/mL
Recovery
Sample Type Mean Recovery (%) Range (%) Serum (n=5) 100 95-109 EDTA Plasma (n=4) 92 87-96 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 100 95-109 Serum (n=5) 100 95-109 EDTA Plasma (n=4) 92 87-96 EDTA Plasma (n=4) 92 87-96
Sensitivity
1.2 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
PTGDS
Protein Name
Prostaglandin-H2 D-isomerase
Gene Name URL
PTGDS
Nucleotide Accession Number
NM_008963

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