APOD ELISA Kit (Mouse)

• Reproducibility:

  Mean Inter-Assay: CV

• Gene ID:

  11815

• Accession Number:

  NP_001288282

• Reactivity:

  Mouse

• Target:

  APOD occurs in the macromolecular complex with lecithin-transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.

• Type:

  ELISA Kit

• Assay Principle:

  Aviva Systems Biology Apod ELISA Kit (Mouse) (OKCA02337) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apod has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Apod is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Apod captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Urine

• Detection Range:

  0.27 - 200 ng/mL

• Recovery:

  Sample Type Mean Recovery (%) Range (%) Serum (n=5) 97 87-103 EDTA Plasma (n=4) 94 91-99 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 97 87-103 Serum (n=5) 97 87-103 EDTA Plasma (n=4) 94 91-99 EDTA Plasma (n=4) 94 91-99

• Sensitivity:

  0.27 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• NCBI Gene Symbol:

  APOD

• Protein Name:

  Apolipoprotein D

• Gene Name URL:

  APOD

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001301353