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APOC2 ELISA Kit (Mouse)

CAT: 0247-OKCA02336-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA02336-96WSize:96 Well
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Reproducibility
Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Aliases
Apo-CII, apoC-II
Gene ID
11813
Accession Number
NP_001264873
Reactivity
Mouse
Target
Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Apoc2 ELISA Kit (Mouse) (OKCA02336) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Apoc2. Sample or standards are added to the wells along with a fixed quantity of HRP-Conjugated Apoc2 Detector Antibody and incubated. The Apoc2 found in the sample or standards competes with immobilized Apoc2 for binding with the HRP-Conjugated Apoc2 Detector Antibody. Wells are washed and an enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product. Addition of acidic stop solution changes the coloration to yellow. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Apoc2captured in the well and inversely proportional to the amount of Apoc2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
18.8 - 1,200 ng/mL
Recovery
Sample Type Mean Recovery (%) Range (%) Serum (n=5) 95 91-99 EDTA Plasma (n=4) 101 94-107 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 95 91-99 Serum (n=5) 95 91-99 EDTA Plasma (n=4) 101 94-107 EDTA Plasma (n=4) 101 94-107
Sensitivity
9.4 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
APOC2
Protein Name
Apolipoprotein C-II
Gene Name URL
APOC2
Nucleotide Accession Number
NM_001277944

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