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EDN1 ELISA Kit (Human)

CAT: 0247-OKCA02265-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA02265-96WSize:96 Well
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Reproducibility
Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Aliases
ET1, HDLCQ7, preproendothelin 1
Gene ID
281137
Accession Number
NP_851353
Reactivity
Human
Target
Endothelins are endothelium-derived vasoconstrictor peptides.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology EDN1 ELISA Kit (Human) (OKCA02265) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EDN1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EDN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard EDN1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Cell Culture Supernates, Urine, Saliva
Detection Range
3.12 - 200 pg/mL
Recovery
Sample Type Mean Recovery (%) Range (%) Serum (n=5) 92 87-97 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 92 87-97 Serum (n=5) 92 87-97
Sensitivity
0.78 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
EDN1
Protein Name
Endothelin-1
Gene Name URL
EDN1
Nucleotide Accession Number
NM_181010

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