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OSM ELISA Kit (Rat)

CAT: 0247-OKCA02245-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA02245-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Variation
CAS Number
7732-18-5
Gene ID
289747
Accession Number
NP_001006962
Reactivity
Rat
Target
Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Osm ELISA Kit (Rat) (OKCA02245) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OSM has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OSM is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample OSM captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma
Detection Range
15.6 pg/mL-1,000 pg/mL
Recovery
Sample Type Mean (%) Range (%) Serum (n=5) 96 92-102 EDTA plasma (n=4) 89 82-93 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 96 92-102 Serum (n=5) 96 92-102 EDTA plasma (n=4) 89 82-93 EDTA plasma (n=4) 89 82-93
Sensitivity
3.9 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
OSM
Protein Name
Oncostatin-M
Gene Name URL
OSM
Nucleotide Accession Number
NM_001006961

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