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IL2 ELISA Kit (Rat)

CAT: 0247-OKCA02236-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA02236-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Variation
CAS Number
7732-18-5
Gene Aliases
TCGF
Gene ID
100302458
Accession Number
NP_001156652
Reactivity
Rat
Target
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology IL2 ELISA Kit (Rat) (OKCA02236) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Urine And Tissue Homogenates
Detection Range
6.25 pg/mL-400 pg/mL
Recovery
Sample Type Mean (%) Range (%) Serum (n=5) 90 85-96 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 90 85-96 Serum (n=5) 90 85-96
Sensitivity
1.56 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
IL2
Protein Name
Interleukin-2
Gene Name URL
IL2
Nucleotide Accession Number
NM_001163180

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