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WNT16 ELISA Kit (Mouse)

CAT: 0247-OKCA02187-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA02187-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Variation
CAS Number
7732-18-5
Gene Aliases
E130309I19Rik
Gene ID
93735
Accession Number
NP_444346
Reactivity
Mouse
Target
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Wnt16 ELISA Kit (Mouse) (OKCA02187) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WNT16 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WNT16 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample WNT16 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates
Detection Range
62.5 pg/mL-4,000 pg/mL
Recovery
Sample Type Mean (%) Range (%) Serum (n=5) 106 101-111 EDTA plasma (n=4) 92 89-96 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 106 101-111 Serum (n=5) 106 101-111 EDTA plasma (n=4) 92 89-96 EDTA plasma (n=4) 92 89-96
Sensitivity
15.6 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
WNT16
Protein Name
Protein Wnt-16
Gene Name URL
WNT16
Nucleotide Accession Number
NM_053116

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