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BMP6 ELISA Kit (Mouse)

CAT: 0247-OKCA02160-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA02160-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Variation
CAS Number
7732-18-5
Gene Aliases
Vg, Vgr1, D13Wsu115, D13Wsu115e
Gene ID
12161
Accession Number
NP_031582
Reactivity
Mouse
Target
Induces cartilage and bone formation.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Bmp6 ELISA Kit (Mouse) (OKCA02160) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMP6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BMP6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample BMP6 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Culture Supernates, Tissue Homogenates
Detection Range
31.25 pg/mL-2,000 pg/mL
Recovery
Sample Type Mean (%) Range (%) Serum (n=5) 100 95-105 EDTA plasma (n=4) 102 96-108 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 100 95-105 Serum (n=5) 100 95-105 EDTA plasma (n=4) 102 96-108 EDTA plasma (n=4) 102 96-108
Sensitivity
7.81 pg/mL.
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
BMP6
Protein Name
Bone morphogenetic protein 6
Gene Name URL
BMP6
Nucleotide Accession Number
NM_007556

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