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ZYX ELISA Kit (Human)

CAT: 0247-OKCA02157-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA02157-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Variation
CAS Number
7732-18-5
Gene Aliases
ESP-2, HED-2
Gene ID
7791
Accession Number
NP_001010972
Reactivity
Human
Target
Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology ZYX ELISA Kit (Human) (OKCA02157) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ZYX has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ZYX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ZYX captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates
Detection Range
23.5 pg/mL-1,500 pg/mL
Recovery
Sample Type Mean (%) Range (%) Serum (n=5) 93 89-97 EDTA plasma (n=4) 88 84-94 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 93 89-97 Serum (n=5) 93 89-97 EDTA plasma (n=4) 88 84-94 EDTA plasma (n=4) 88 84-94
Sensitivity
5.8 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
ZYX
Protein Name
Zyxin
Gene Name URL
ZYX
Nucleotide Accession Number
NM_001010972

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