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PPY ELISA Kit (Rat)

CAT: 0247-OKCA01914-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA01914-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Pancreatic hormone
Gene Aliases
PNP, pancreatic polypeptide Y
Accession Number
XP_002719475.3
Reactivity
Rat
Target
Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology PPY ELISA Kit (Rat) (OKCA01914) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PPY has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PPY is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PPY captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates
Detection Range
0.78 - 50 pg/mL
Recovery
Sample Mean Recovery (%) Range (%) Serum (n=5) 90 83-94 EDTA Plasma (n=4) 100 91-104 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 90 83-94 Serum (n=5) 90 83-94 EDTA Plasma (n=4) 100 91-104 EDTA Plasma (n=4) 100 91-104
Sensitivity
0.2 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
PPY
Protein Name
Pancreatic hormone
Gene Name URL
PPY
Nucleotide Accession Number
XM_002719429.3