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SDC4 ELISA Kit (Mouse)

CAT: 0247-OKCA01781-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA01781-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Syndecan 4
Gene Aliases
S, S4, Syn, ryud, Synd4, syndec, AA959608, AW108331, ryudocan, syndecan-4
Gene ID
20971
Accession Number
NP_035651.1
Reactivity
Mouse
Target
Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Sdc4 ELISA Kit (Mouse) (OKCA01781) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sdc4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Sdc4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Sdc4 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
3.12 - 200 pg/mL
Recovery
Sample Mean Recovery (%) Range (%) Serum (n=5) 89 84-95 EDTA Plasma (n=4) 97 92-101 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 89 84-95 Serum (n=5) 89 84-95 EDTA Plasma (n=4) 97 92-101 EDTA Plasma (n=4) 97 92-101
Sensitivity
0.78 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
SDC4
Protein Name
Syndecan-4
Gene Name URL
SDC4
Nucleotide Accession Number
NM_011521.2

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