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SPON1 ELISA Kit (Mouse)

CAT: 0247-OKCA01777-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA01777-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Spondin 1, (f-spondin) extracellular matrix protein
Gene Aliases
F, FSP, AI666765, AW455831, BC020531, D330035F22Rik
Gene ID
233744
Accession Number
NP_663559.1
Reactivity
Mouse
Target
Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Spon1 ELISA Kit (Mouse) (OKCA01777) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Spon1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Spon1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Spon1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.156 - 10 ng/mL
Recovery
Sample Mean Recovery (%) Range (%) Serum (n=5) 102 97-106 EDTA Plasma (n=4) 87 83-92 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 102 97-106 Serum (n=5) 102 97-106 EDTA Plasma (n=4) 87 83-92 EDTA Plasma (n=4) 87 83-92
Sensitivity
0.039 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
SPON1
Protein Name
Spondin-1
Gene Name URL
SPON1
Nucleotide Accession Number
NM_145584.2

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