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PAH ELISA Kit (Mouse)

CAT: 0247-OKCA01742-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA01742-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Phenylalanine hydroxylase
Gene Aliases
AW106920
Gene ID
18478
Accession Number
NP_032803.2
Reactivity
Mouse
Target
Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Pah ELISA Kit (Mouse) (OKCA01742) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pah has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pah is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Pah captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and cell lysates
Detection Range
23.5 - 1,500 pg/mL
Recovery
Sample Mean Recovery (%) Range (%) _x005F Serum (n=5) 86 83-89 _x005F EDTA Plasma (n=4) 89 85-96 _x005F Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 86 83-89 Serum (n=5) 86 83-89 EDTA Plasma (n=4) 89 85-96 EDTA Plasma (n=4) 89 85-96
Sensitivity
5.8 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
PAH
Protein Name
Phenylalanine-4-hydroxylase
Gene Name URL
PAH
Nucleotide Accession Number
NM_008777.3

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