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IL4I1 ELISA Kit (Mouse)

CAT: 0247-OKCA01705-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA01705-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Interleukin 4 induced 1
Gene Aliases
H4, Fig, H-4, H46, LAO, Fig1, H-46, LAAO, mIL4I1, Fig1-ps
Gene ID
14204
Accession Number
NP_001164495.1
Reactivity
Mouse
Target
Lysosomal L-amino-acid oxidase with highest specific activity with phenylalanine. May play a role in lysosomal antigen processing and presentation.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Il4i1 ELISA Kit (Mouse) (OKCA01705) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il4i1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Il4i1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Il4i1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates
Detection Range
15.6 - 1,000 pg/mL
Recovery
Sample Mean Recovery (%) Range (%) Serum (n=5) 103 94-107 EDTA Plasma (n=4) 98 90-102 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 103 94-107 Serum (n=5) 103 94-107 EDTA Plasma (n=4) 98 90-102 EDTA Plasma (n=4) 98 90-102
Sensitivity
3.9 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
IL4I1
Protein Name
L-amino-acid oxidase
Gene Name URL
IL4I1
Nucleotide Accession Number
NM_001171024.1

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